Although structural details of the cytoskeleton can be revealed by electron microscopy there are limitations that must be considered. Since samples in the electron microscope must be viewed in a vacuum we must immobilise them by chemical fixation or freezing before electron microscopy. For optimal visualization of the cytoskeleton, the cell membrane, organelles and soluble components of the cytoplasm are preferentially removed to expose it. This is best achieved by adding a detergent to the fixation mixture. Details of the procedures we use are presented in Auinger and Small (2008).
- Auinger S., Small, J.V. (2008). Correlated light and electron microscopy of the cytoskeleton. Methods Cell Biol. 257–272.